Genetics of human superoxide dismutase

Human Erythrocyte Superoxide Dismutase Encapsulated in Positively Charged Liposomes

      Superoxide dismutase (SOD) is an important antioxidant that protects many types of cells from the free radical damage. One of the possible ways for the use of SOD is its incorporation in liposomes. The aim of this study was to investigate the effect of cationic phospholipids on the entrapment of human erythrocyte superoxide dismutase (Cu/Zn SOD) in liposomes. Also, in the present study, w...

Superoxide dismutase Superoxide dismutase

Subunit structure of human superoxide dismutase.

A subunit structure for human superoxide dismutase has been demonstrated by sedimentation equilibrium, agarose gel filtration, and sodium dodecyl sulfate polyacrylamide gel electrophoretic studies. This protein appears to be composed of 2 subunits, not linked by covalent bonds, each with a molecular weight about 16,000. Dissociation of the subunits is facilitated by alkylation of the two sulfhy...

Further characterization of human erythrocyte superoxide dismutase.

1. A simplified procedure for the preparation of highly purified human superoxide dismutase from erythrocytes was developed which avoided extremes of pH and ionic strength and the use of organic solvents; the properties of human and bovine proteins, prepared by the method, were compared. 2. Using the two dimensional electrophoretic procedure of O'Farrell, the human superoxide dismutase was foun...

Mn-superoxide dismutase in human dermal fibroblasts

To determine the effect of oxidative stress on expression of extracellular superoxide dismutase (EC-SOD), CuZn-SOD and Mn-SOD, two fibroblast lines were exposed for periods of up to 4 days to a wide concentration range of oxidizing agents: xanthine oxidase plus hypoxanthine, paraquat, pyrogallol, anaphthoflavone, hydroquinone, catechol, Fe2l ions, Cu2+ ions, buthionine sulphoximine, diethylmale...